Suppression of Bacterial Wilt in Tomato Plant Using Pseudomonas putida P84

Seo, Sang Tae; Park, Jong Han; Kim, Kyung Hee; Lee, Sang Hyun; Oh, Eun Sung; Shin, Sang Chul

Research in Plant Disease 2008;14(1):32-36.
Published online April 30, 2008.

Pseudomonas putida P84 균주를 이용한 토마토 풋마름병의 억제

서상태, 박종한, 김경희, 이상현, 오은성, 신상철

Suppression of Bacterial Wilt in Tomato Plant Using Pseudomonas putida P84

Sang Tae Seo, Jong Han Park, Kyung Hee Kim, Sang Hyun Lee, Eun Sung Oh, Sang Chul Shin

Abstract

Bacterial wilt caused by Ralstonia solanacearum has become a severe problem on tomato in Korea and no effective control measures are available yet. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. A rhizobacterial population of 150 Pseudomonas strains, isolated from the rhizosphere soil of various plants grown at different sites, was screened for 2,4-diacetylphloroglucinol producing gene (phlD) by PCR. Two strains (P83 and P84) among them were found to be phlD positive. When the isolates were analysed by 16S rDNA (Sensu Stricto), all isolates yielded amplified products of 1,018 bp. Of the 150 isolates of Pseudomonas spp., a bacterial strain P. putida P84 isolated from tomato rhizosphere showed to suppress a wide range of phytopathogenic bacteria in vitro. The best source of carbon for P84 strain were glucose, arabinose, inositol and melibiose. In greenhouse experiments, P84 strain suppressed the development of bacterial wilt in tomato with a control value of 60%.

Key Words: Bacterial wilt, Biocontrol, Ralstonia solanacearum, Tomato