Research in Plant Disease 2008;14(1):15-20.
Published online April 30, 2008.
잡초에서 분리한 3종 Cucumber mosaic virus의 동정과 특성
이혁근, 김성률, 전용운, 권순배, 류기현, 최장경
 
Identification and Characterization of Three Isolates of Cucumber mosaic virus Isolated from Weed Hosts
Hyeok Geun Lee, Sung Ryul Kim, Yong Woon Jeon, Soon Bae Kwon, Ki Hyun Ryu, Jang Kyung Choi
Abstract
Three isolates of Cucumber mosaic virus (CMV) were isolated from weed hosts showing typical mosaic symptoms, and some properties of the viruses were investigated. CMV isolates, designated as Is-CMV, Jd-CMV and Pla-CMV from Isodon inflexus, Jeffersonia dubia and Phryma leptostachya var. asiatica, respectively, were identified and characterized by biological reaction in several host plants, serological property, dsRNA analysis, reverse transcription-polymerase chain reaction (RT-PCR), restriction fragment-length polymorphism (RFLP). All isolates systemically infected in Nicotiana benthamiana, Cucurbita pepo cv. Black beauty and Cucumis sativus, and did not reveal any differences in these host plants between the isolates. However, remarkable difference in the symptoms was found between the CMVs in Capsicum annuum. Is-CMV induced an asymptomatic symptoms, while Jd-CMV and Pla-CMV produced severe mosaic symptoms in C. annuum plants. In dsRNA analysis, all isolates revealed four major bands with estimated molecular size of 3.4, 3.2, 2.1 and 1.0 kbp. The cDNAs of coat protein gene of the isolates were amplified by RT-PCR using a genus-specific single pair primers that designed to amplify a DNA fragment of approximately ranging from 938 to 966 bp. By restriction mapping analysis using RFLP of the RT-PCR products as well as by serological properties of gel diffusion test, the CMV isolates belong to a typical members of CMV subgroup IA. This is the first report on the occurrence of CMV in the three weed hosts.
Key Words: Cucumber mosaic virus, Host reaction, RFLP, Subgroup IA, Weed hosts


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