Research in Plant Disease 2007;13(3):170-176.
Published online December 30, 2007.
인삼 잿빛곰팡이병균의 procymidone에 대한 감수성 변화와 carbendazim/diethofencarb 합제와의 다중 저항성
이선욱, 김주형, 민지영, 배영석, 김흥태
Monitoring for the Resistance of Botrytis cinerea Causing Ginseng Gray Mold to Procymidone and Its Multiple resistance with the Mixture of Carbendazim/Diethofencarb
Seon Wook Lee, Joo Hyung Kim, Ji Young Min, Young Seok Bae, Heung Tae Kim
Effects of fungicides on the mycelial growth of Botrytis cinerea isolated from ginseng leaves were investigated by an agar dilution method. By using a agar dilution method, it was investigated the effect of fungicides, procymidone, carbendazim and the mixture with both of carbendazim and diethofencarb, on the mycelial growth of Botrytis cinerea isolates, which were isolated from infected leaves of ginseng in 2005 and 2006. With MIC (minimum inhibiton concentration) of procymidone against B. cinerea, pathogens were divided into two groups. While one showed the low MIC between 0.8 and 4.0 μg/ml, the other showed higher MIC above 20 μg/ml. In terms of the inhibition ratio of mycelial growth at the indicated concentration of procymidone, isolates of B. cinerea were divided into three groups; the sensitive, the intermediate resistant, and the resistant group. Each group was differentiated by EC50; the sensitive group showed below 2.0 μg/ml, the intermediate resistant group between 2.0 to 5.0 μg/ml, and resistant group above 5.0 μg/ml. Compared with the ratio of resistant isolates of B. cinerea in 2005, the ratio in 2006 increased from 19.3% to 27.5%. Furthermore, the average EC50 value of them increased from 10.0 μg/ml in 2005 to 237.3 μg/ml in 2006. The ratio of isolates showing the multiple resistance between procymidone and carbendazim was 40.2%, whereas the ratio was 4.0% showing the multiple resistance in the mixture.
Key Words: Botrytis cinerea, Fungicide resistance, Ginseng gray mold, Procymidone

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