Research in Plant Disease 2007;13(3):145-151.
Published online December 30, 2007.
PCR Assay 이용 콩 종자에서 Xanthomonas axonopodis pv. glycines 검출 및 종자오염 조사
홍성준, 홍연규, 이봉춘, 임미정, 윤영남, 황재복, 송석보, 박성태
Detection of Xanthomonas axonopodis pv. glycines and Survey on Seed Contamination in Soybean Seeds Using PCR Assay
Sung Jun Hong, Yeon Kyu Hong, Bong Choon Lee, Mi Jung Lim, Young Nam Yoon, Jae Bok Hwang, Seok Bo Song, Sung Tae Park
Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.
Key Words: PCR assay, Soybean seed, Xanthomonas axonopodis pv. glycines

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