Research in Plant Disease 2007;13(3):137-144.
Published online December 30, 2007.
국내 포도나무 혹병(Agrobacterium vitis) 균주의 유전적 다양성
김종군, 최재을, 강희완
 
Genetic Diversity of Agrobacterium vitis Strains in Korea
Jong Kun Kim, Jae Eul Choi, Hee Wan Kang
Abstract
Fifty nine strains of Agrobacterium vitis, the causal agent of crown-gall disease on grapevine, originating from different geographical regions and 16 grapevine cultivars including 35 Kyoho cultivar of Korea, were characterized by PCR polymorphic analysis using Universal Rice Primer(URP). Of 12 URP primers, primers URP1F, URP2R, URP2F, and URP4R, URP17R were available for detecting PCR polymorphic bands among the A. vitis strains. PCR polymorphic bands produced by primers URP2F and URP17R were profiled to 12 strain types. A. vitis strains originated from Kyoho cultivar of grapevine showed relatively simple genetic diversity of the four PCR types, while the A. vitis strains originated from other grapevine cultivars and type culture strains showed various genetic diversity with 8 types. Unweighted Pair-Group Method with Arithmetic mean(UPGMA) cluster analysis using the URP-PCR polymorphic bands showed 59 A. vitis strains are genetically clustered into large seven groups.
Key Words: Agrobacterium vitis, Crown gall, Grapevine, Genetic diversity, Pathogenicity, PCR polymorphic


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