Research in Plant Disease 2006;12(2):144-147.
Published online August 30, 2006.
Pepper mild mottle virus에 대한 난황항체의 생산과 혈청학적 진단에의 활용
한정헌, 이철호, 김영호, 나용준
Production of Egg Yolk Immunoglobulin and Its Application for Pepper mild mottle virus in Serological Tests
Jung Heon Han, Cheol Ho Lee, Young Ho Kim, Yong Joon La
Egg yolk immunoglobulin (IgY) is much widely used in medical fields, but its use in serology of plant viruses is much limited. We produced an IgY against pepper mild mottle virus (PMMoV) and applied it to several serological tests. Polyclonal antibodies were obtained from the egg yolk of chicken immunized with a total of 2mg of purified PMMoV over 2 months. The titers of antibodies were measured with the ring-test over six months after the first injection. The highest titers of IgY was 1/2,560 at 2 months after the first injection. Approximately 60-80 mg of IgY were obtained from one egg yolk. Using the IgY, 1 ng/ml of purified PMMoV was detected with the indirect ELISA. Gelrite gel double diffusion test, ELISA and tissue immuno-binding assay employing IgY gave similar sensitivity and specificity to those of IgG developed in rabbit. Therefore, the IgY which can be obtained in large quantities from a chicken, might be useful for the antibody production and the serology of plant viruses.
Key Words: Egg yolk immunoglobulin, IgY, Antibody, Peper, Pepper mild mottle virus, PMMoV
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