Research in Plant Disease 2006;12(2):85-90.
Published online August 30, 2006.
차나무 겹둥근무늬병 방제용 미생물제제 개발을 위한 길항세균Bacillus subtilis BD0310의 대량배양 최적조건
김경희, 오순옥, 허재선, 염규진, 고영진
Optimum Cultivation Conditions for Mass Production of an Antagonistic Bacterium Bacillus subtilis BD0310 for Development of a Microbial Agent Controlling Gray Blight of Tea Plants
Gyoung Hee Kim, Soon Ok Oh, Jae Seoun Hur, Kue Jin Yum, Young Jin Koh
Bacillus subtilis BD0310 isolated from tea leaves was used for the development of a biofungicide against Pestalotiopsis longiseta causing gray blight of tea plants. The optimum growth conditions were investigated for the mass cultivation of the microbial agent. The optimum temperature and cultivation time were determined as 12~24 hours at 30oC and the optimum initial pH was pH 7.0 in nutrient broth. Among the tested carbon sources of fructose, galactose, glucose, glycerol, inositol, lactose, maltose, sorbitol and starch, maltose and inositol were found to highly increase antifungal activity of the microbial agent against P. longiseta. Yeast extract and tryptone apparently increased antifungal activity of the microbial agent among the tested nitrogen sources of casein, tryptone, malt extract, yeast extract and (NH4)2SO4. The results will make a contribution to mass production of the antagonistic bacterium Bacillus subtilis BD0310 for development of a microbial agent controlling gray blight of tea plants.
Key Words: Bacillus subtilis BD0310, Biocontrol, Gray blight, Microbial agent, Pestalotiopsis Longiseta

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