Rhizoctonia solani에 의한 결구상추 밑둥썩음병 방제균주 Stenotrophomonas maltophilia BW-13의 분리 및 동정 |
김한우, 박종영, 김현주, 이광렬, 이진우, 최우봉, 이선우, 문병주 |
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Isolation and Identification of Stenotrophomonas maltophilia BW-13 Active Against Rhizoctonia solani Causing Crisphead Lettuce Bottom Rot |
Han Woo Kim, Jong Young Park, Hyun Ju Kim, Kwang Youll Lee, Jin Woo Lee, Woo Bong Choi, Seon Woo Lee, Byung Ju Moon |
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Abstract |
In a course of searching for biofungicide to control crisphead lettuce bottom rot caused by Rhizoctonia solani, we have isolated an antagonistic bacterium from lettuce rhisophere soil. A total of 702 bacterial isolates were isolated and tested for in vitro growth inhibition of R. solani. Seven strains appeared to have strong antagonistic effect against R. solani in in vitro growth inhibition assay. In the pot experiments, a strain BW-13 showed the most potent disease control effect on the both lettuce seedlings and adults plants. Therefore, the BW-13 was selected as a biocotrol candidate against crisphead lettuce bottom rot. Based on its morphology, physiological characteristics, and 16S rRNA gene analysis, the BW-13 was finally identified as Stenotrophomonas maltophilia. This study indicated that S. maltophilia BW-13 could be used as a biocontrol agent to control crisphead lettuce bottom rot. |
Key Words:
Antagonistic bacteria, Bottom rot, Rhizoctonia solani, Stenotrophomonas maltophilia |
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