Novel Pathogenic Strain of Watermelon mosaic virus Occurred on Insam (Panax ginseng)

Research in Plant Disease 2013;19(4):331-337.
Published online December 30, 2013.

인삼(Panax ginseng)에 발생한 Watermelon mosaic virus의 새로운 병원성 계통

정원권 ( Won Kwon Jung ) , 남문 ( Moon Nam ) , 이주희 ( Joo Hee Lee ) , 박충열 ( Chung Youl Park ) , 김병훈 ( Byoung Hoon Kim ) , 박은혜 ( Eun Hye Park ) , 이민아 ( Min A Lee4 ) , 김미경 ( Mi Kyeong Kim ) , 최홍수 ( Hong Soo Choi ) , 이준성 ( Jun Seong Lee ) , 김정수 ( Jeong Soo Kim ) ,

Novel Pathogenic Strain of Watermelon mosaic virus Occurred on Insam (Panax ginseng)

Won-Kwon Jung1†, Moon Nam2†, Joo Hee Lee3, Chung Youl Park4, Byoung Hoon Kim4, Eun Hye Park4, Min-A Lee4, Mi-Kyeong Kim3, Hong-Soo Choi3, Jun Seong Lee3, Jeong-Soo Kim5, Jin Kook Choi1, Tae Ryong Kwon1, Key-Woon Lee4 and Su-Heon Lee4*

¹Punggi Ginseng Experiment, Gyeongsangbukdo Agricultural Research and Extension Services, Youngju 750-871, Korea
²Institute of Plant Medicine, Kyungpook National University, Daegu 702-701, Korea
³Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Korea
⁴School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea
⁵Plant Medicine Major, Andong National University, Andong 760-749, Korea

Received: October 31, 2013 Revised: November 03, 2013 Accepted: November 21, 2013

Abstract

A disease, supposedly caused by a virus, was observed from Insam (Panax ginseng) fields of Punggi in year 2006. It has long believed to be a physiological disorder. However, the incidence of the disease has increased every year. When several samples were observed under electron microscope, filamentous virus-like particles were observed. The nucleotide sequences of the virus were analyzed by RT-PCR with specific primer sets derived from the results of DNA chip. The results indicated that the disease was caused by Watermelon mosaic virus (WMV). It revealed that the result of the biological assay by the virus was different from that of WMV previously found in other crops. Therefore, this is the first report that WMV causes the disease in P. ginseng and the virus is named to be WMV-Insam.

Key Words: DNA chip, Insam, Panax ginseng, Potyvirus, Watermelon mosaic virus