| 근권세균을 전 접종한 감귤에서 감귤 더뎅이병 억제 효과 및 기작 |
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현재욱, 김소연, 전용철 |
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| Suppression Effect and Mechanism of Citrus Scab in the Citrus Pre-inoculated with Rhizobacterial Strains |
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Jae Wook Hyun, So Yeon Kim, Yong Chull Jeun |
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| Abstract |
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Elsinoe fawcettii is one of major pathogenic fungi which cause citrus scab diseases, resulting in fruit blemishes that reduce the economic value of fruit. By increasing interest to safe products of crops, the alternative methods of disease control is highly required. We investigated whether the 215 bacterial strains isolated from Jeju Island possess antifungal effect or suppression effect on the symptom development by Elsinoe fawcettii on citrus. Among them, three bacterial strains THJ 609-3, MRL408-3, and TRH423-3 that exhibited antifungal capacity against Elsinoe fawcettii were selected. To illustrate the disease suppression mechanism, pre-inoculation with the selected bacterial strains was carried out whether could suppress the citrus crab on the leaves. The observation with a fluorescence microscope revealed that the selected bacteria could decrease the number of fungal spores. The ratio of germ tube formation was also decreased by the selected bacterial strains at one day after fungus challenge. The strain THJ 609-3 was identified as Pseudomonas putida as a result of analyzing the internal transcript spaces of the rhizobacterial rDNA. The strains MRL 408-3 and TRH 423-3 were identified as Burkholderia gladioli. Our results may be valuable when the selected rhizobacterial strains used as the environment-friendly microbe for biological control on citrus scab caused by Elsinoe fawcettii. |
| Key Words:
Antifungal activity, Biological control, Fluorescent microscopy |
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