Occurrence of Pome Fruit Viruses on Pear Trees (Pyrus pyrifolia) in Korea

Cho, In Sook; Kim, Dae Huyn; Kim, Hyun Ran; Chung, Bong Nam; Cho, Jeom Doeg; Choi, Gug Seoun

Research in Plant Disease 2010;16(3):326-330.
Published online December 30, 2010.

국내에서 발생하는 배나무 바이러스병

조인숙, 김대현, 김현란, 정봉남, 조점덕, 최국선

Occurrence of Pome Fruit Viruses on Pear Trees (Pyrus pyrifolia) in Korea

In Sook Cho, Dae Huyn Kim, Hyun Ran Kim, Bong Nam Chung, Jeom Doeg Cho, Gug Seoun Choi

Abstract

Three pome fruit viruses, Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASPV) and Apple stem pitting virus (ASGV) were detected in pear trees (Pyrus pyrifolia) using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in Ansung, Naju and Ulsan provinces of Korea. Infection rate of three viruses was 35.2% from 452 leaf samples of the three cultivars of pear trees. Also, each of three viruses was detected by reverse transcription polymerase chain reaction (RT-PCR) for a limited number of samples. Infection rate of three viruses was 86.3% from 233 leaf samples of the three pear cultivars. The virus infection rates by RT-PCR were much higher than ELISA. ASGV was prevailing on pear with 74.2%, whereas ASPV and ACLSV were found in 34.8% and 0.4% of tested samples, respectively. Symptoms caused by ASGV showed black spots of infected Niitaka cultivar leaves. The ACLSV, ASPV and ASGV isolates showed 83~94% sequence identity at a nucleotide level to other pome fruit virus isolates when analyzed by NCBI BLAST. Pome fruit viruses occurring in pear were ACLSV, ASPV and ASGV. This is the first report of pear trees infected ASPV in Korea.

Key Words: ACLSV, ASGV, ASPV, Nucleotide sequence, Pear (Pyrus pyrifolia)