고추 유묘에 대한 Colletotrichum acutatum의 병원성과 탄저병 발생 |
한경숙, 박종한, 한유경, 황정환 |
|
Pathogenicity and Occurrence of Pepper Seedling Anthracnose Caused by Colletotrichum acutatum |
Kyung Sook Han, Jong Han Park, You Kyoung Han, Jung Hwan Hwang |
|
Abstract |
In 2008, leaf rot and blight on pepper seeding ("Dokya-chungchung") occurred in a pepper farm at Hwaseong-si, Gyeonggi-do, Korea. The typical symptom is water-soaking and dark brown leaf blight at edges and tips of leaves. The fungal colonies isolated from infected tissues were pinkish at first and turned gradually to gray. Conidia were fusiform, non-septum, and 8.1-17.0×2.0-3.8 μm in size. Several specific PCR primers derived from the sequence of the internal transcribed spacer (ITS) region of the rDNA, such as CaINT, CgINT and CcINT were used for the identification of the fungal pathogen. The C. acutatum-specific primer CaINT was amplified single fragment of 496 bp that discriminated C. acutatum from the other species. The pathogenicity test was performed on seedlings and fruits of red pepper. On the basis of the morphological, molecular characteristics and pathogenicity test, we identified as Colletotrichum acutatum. This is the first report on leaf rot and blight on pepper seedling caused by C. acutatum in Korea. |
Key Words:
Anthracnose, Colletotrichum acutatum, Pepper, Seedling |
|