Streptomycin Resistant Genes of Pseudomonas syringae pv. syringae, the Causal Agent of Bacterial Blossom Blight of Kiwifruit

Park, So Yeon; Han, Hyo Shim; Lee, Young Sun; Koh, Young Jin; Jung, Jae Sung

Research in Plant Disease 2007;13(2):88-92.
Published online August 30, 2007.

참다래 꽃썩음병 병원세균(Pseudomonas syringae pv. syringae)의 스트렙토마이신 저항성 유전자

박소연, 한효심, 이영선, 고영진, 정재성

Streptomycin Resistant Genes of Pseudomonas syringae pv. syringae, the Causal Agent of Bacterial Blossom Blight of Kiwifruit

So Yeon Park, Hyo Shim Han, Young Sun Lee, Young Jin Koh, Jae Sung Jung

Abstract

A total of 41 Pseudomonas syringae pv. syringae, the causal agent of bacterial blossom blight, were isolated from kiwifruit plants in Korea. Among them, two strains showing streptomycin resistance were examined to investigate the structure of resistant determinants by PCR and nucleotide sequence analysis. PCR results suggested that the streptomycin resistance is mediated by strA-strB genes carried on Tn5393a. Insertion sequences, IS6100 and IS1133, which were located within or downstream of tnpR gene in Xanthomonas campestris and Erwinia amylovora were not found. Nucleotide sequences of strA-strB were 100% identical with Tn5393a. Two stretomycin resistant strains had three plasmids. Southern blot hybridization using strAstrB probe indicated that the resistant genes were carried on a 100 kb plasmid.

Key Words: Blosom blight, Kiwifruit, Pseudomonas syringae pv. syringae, Streptomycin resistance