Occurrence of Rice black-streaked dwarf fijivirus in Maize

Lee, Bong Choon; Hong, Yeon Kyu; Hong, Sung Jun; Park, Sung Tae

Research in Plant Disease 2006;12(1):62-64.
Published online April 30, 2006.

옥수수의 벼검은줄오갈병

이봉춘, 홍연규, 홍성준, 박성태

Occurrence of Rice black-streaked dwarf fijivirus in Maize

Bong Choon Lee, Yeon Kyu Hong, Sung Jun Hong, Sung Tae Park

Abstract

July in 2005, we collected infected maize plant that showing stripe dwarf disease on maize leaf in Jeonbuk provinces including Gochang-gun and conducted genomic dsRNA extraction and RT-PCR. Genomic dsRNA was extracted directly in infected maize plant and electrophoresis in agarose gel. We confirmed 10 segments of genomic dsRNA. We conducted RT-PCR by genomic dsRNA and specific primer of S7, S8 and S10. As a result, specific band of expected size was confirmed respectively. In the results of dsRNA and RT-PCR analysis, we confirmed Rice black-streaked dwarf fijivirus (RBSDV) from naturally infected maize plant. Occurrence of RBSDV of maize plant was dealt 22 ha``s damage in maize field. The occurrence rate was 80% in a lot of places of disease.

Key Words: Genomic dsRNA, Rice black-streaked dwarf fijivirus, RT-PCR